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14I2I05 - Molecular-genetic methods in veterinary medicine

Course specification
Course titleMolecular-genetic methods in veterinary medicine
Acronym14I2I05
AcronymVeterinary Medicine
Module
Type of studyIntegrated academic studies
Lecturer (for classes)
  1. dr Jevrosima Stevanović
  2. dr Zoran Stanimirović
  3. dr Ninoslav Đelić
Lecturer/Associate (for practice)
  1. Uroš Glavinić
Lecturer/Associate (for OTC)
    ESPB2.0Status
    ConditionPassed examinations in Zoology and Biochemistry
    The goalThe subject aims to provide students with basic knowledge about the importance and application of molecular diagnostic methods in veterinary medicine; to explain the basic principles of methods based on the analysis of DNA and RNA and its applications in veterinary diagnostics; to introduce students with the work rules in the laboratory of Molecular Genetics.
    The outcomeThe student should know the basic principles of methods used in molecular genetics. Student should accept the rules of bah and work in the laboratory of Molecular Genetics. The student should learn the most commonly applied molecular techniques and their application in veterinary medicine. The student should understand procedures of basic molecular methods. Student should be able to perform basic analysis of the sampled animal material applying appropriate molecular techniques (sex determination and parentage identification in animals, genotype analyses, the detection and identification of animal pathogens).
    Contents
    Contents of lecturesThe basic principles of molecular genetics: the structure of DNA and RNA; central dogma of molecular biology (1). Necessary conditions and rules in the laboratory for molecular genetics of animals (2). Molecular genetic techniques and their application in veterinary medicine (2). Preparation procedures for performing molecular analysis. Extraction of DNA and RNA (2). Amplification of nucleic acids in vitro by polymerase chain reaction (PCR) (2). The technique of reverse transcription. Quantification of the obtained PCR product (2). Basics of sequencing and its application in veterinary medicine (2). The use of molecular methods in sex determination in birds, genotype analyses, the detection and determination of the causative agents in diseased animals. Diagnostic of genetic disease (2).
    Contents of exercisesLaboratory of Molecular Genetics. Rules of behaviour and safety measures in the laboratory of Molecular Genetics (1). The samples manipulation: receiving and storing samples for molecular analysis. Pipetting practise (2). Demonstration of techniques used in molecular methods (2). Method for the extraction of DNA using commercial kits. The process of RNA extraction using commercial kits (2). Setting- up PCR reactions (preparation of PCR master mix and adding sample of extracted DNA/RNA ) (2). The technique of reverse transcription; visualisation of products obtained using PCR, RT-PCR and Real-time PCR (2). Sequencing method (2). Presentation of results obtained by molecular analysis: sex determinatin in birds, detection and determination of the causative agents in diseased animals (Nosema sp., Babesia sp., Dirofilaria sp., Parvovirus, Morbillivirus, bee viruses) (2).
    Literature
    1. Djelic N, Stanimirovic Z: Principles of genetics, Faculty of Veterinary Medicine, University of Belgrade, Elit - Medica, Belgrade, 2004.
    2. Kulić M, Z Stanimirović, Delic N, Novakovic M: Human genetics. Faculty of Medicine Foča, University of East Sarajevo, Foča, 2010.
    3. Stanimirović Z, Stevanović J: Applications of molecular-genetic analyses in veterinary medicine. Proceedings of the XXXIII Seminar for inovation of knowledge of veterinarians, Feb 24th, pp 17-33, Belgrade, Serbia, 2012.
    4. Stanimirović Z, Simeunović P, Vučićević M, Davitkov D, Stanišić Lj, Maletić M, Gajić B, Glavinić U, Stevanović J: Application of molecular genetic analyses in forensics and diagnosis in domestic and wild animals. Proceedings of the XXXVI Seminar for inovation of knowledge of veterinarians, Feb 20th, pp 137-146, Belgrade, Serbia, 2014.
    5. Primrose SB, Twyman R: Principles of gene manipulation and genomics. Wiley - Blackwell , 2009. (Original title)
    6. Pestana EA, Belak S, Diallo A, Crowther JR, Viljoen GJ: Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications. Springer, London 2010. (Original title)
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    11
    Methods of teachingTheoretical instruction through interactive teaching with applicatrion of audio-visual methods (PowerPoint Presentations, films). Demonstration of procedures and practical work in the Laboratory of animal genetics at the Department of Biology, Faculty of Veterinary Medicine. Students' individual work in the Laboratory.
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lectures20Test paper0
    Practical lessons50Oral examination30
    Projects0
    Colloquia0
    Seminars0