14D2I32 - Behaviour and molecular ecology of bees

Course specification
Course titleBehaviour and molecular ecology of bees
Type of studyPhD academic studies
Lecturer (for classes)
  1. dr Zoran Stanimirović
  2. dr Jevrosima Stevanović
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ConditionEnrolled semester in which the subject is attended
      The goalThe subject aims to provide students the knowledge about application of molecular genetics methods in exploration of ecology, behaviour and protection of honey bees.
      The outcomeStudent should become capable for application of molecular genetic methods in analyses of behaviour and ecology of bees, as well as in exploration of bee pathogens aimed to maintain health honey bee colonies.
      Contents of lecturesTheoretical instructions: Genetic analyses of honey bees. Mitochondrial and nuclear DNA – evaluation of genetic diversity and protection of honey bee ecotypes. Gene flow as an indicator of migratory beekeeping, commercial breeding and queen introduction. Molecular genetics in bee breeding and selection programs aimed to increase disease resistance. Behavioural genetics of bees. Heritability coefficient of hygienic and grooming behaviours in suppression of honey bee mite reproduction. Molecular characterization and typization of pathogens of bees and bee brood.
      Contents of exercisesIndependent research activities: DNA isolation, amplification of conserved regions of mitochondrial DNA, amplification of nuclear DNA microsatellites (PCR, real-time PCR, RT-PCR, PCR-RFLP, LAMP), visualisation of results by gel electrophoresis and DNA sequencing, analysis of the obtained results with an aim to analyse genome and molecular diversity of bees. Molecular detection and identification of bee pathogens and parasites.
      1. Stanimirović Z, Soldatović B, Vučinić M: Bee biology-Honey bee, Medicinska knjiga-Medicinske komunikacije, Beograd, 2000.
      2. Baker A (ed): Molecular Methods in Ecology, Blackwell Science, 2000. (Original title)
      3. Hoy MA: Insect Molecular Genetics: An Introduction to Principles and Applications, 2nd ed. Academic Press/Elsevier, 2003. (Original title)
      4. Behura SK: Molecular marker systems in insects: current trends and future avenues. Molecular Ecology 15 (11) 3087–3113, 2006. (Original title)
      Number of hours per week during the semester/trimester/year
      LecturesExercisesOTCStudy and ResearchOther classes
      Methods of teachingTheoretical instruction through preparation of seminary elaborate, public interpretation and critical discussion. Independent research activities: performing experiment, analysis and discussion of the results.
      Knowledge score (maximum points 100)
      Pre obligationsPointsFinal examPoints
      Activites during lectures10Test paper70
      Practical lessonsOral examination