14D2I06 - Methods of microbiological and immunobiological diagnostics

Course specification
Course titleMethods of microbiological and immunobiological diagnostics
Type of studyPhD academic studies
Lecturer (for classes)
  1. prof. dr Nenad Milić
  2. prof. dr Dejan Krnjaić
  3. prof. dr Dušan Mišić
  4. prof. dr Jakov Nišavić
  5. prof. dr Marina Radojičić
  6. doc. dr Andrea Radalj
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ConditionEnrolled in 4rd semester
      The goalAcquiring knowledge on standard microbiological and immunological methods for identification of isolated microorganisms and the methods of determination of immunological status of infected and immunized animals.
      The outcomeStudents should be able to properly perform sampling, packaging and shipping of suspected material, to learn to use the microscope and carry out microbiological staining methods in order to morphologically identify microorganisms from suspected material. Students should be capable of performing the isolation of microorganisms in pure culture and their identification by applying standard diagnostic methods as well as automatic identification systems and to carry out basic serological and virological diagnostic methods.
      Contents of lecturesMethods of morphological examination of pathogenic microorganisms. Sterilization. Nutrient media. Sample processing. Biochemical reactions. Methods of isolation and identification of aerobic, anaerobic and capnophylic bacteria. Methods of isolation and identification of fungi. Serological reactions. Molecular diagnostic methods.
      Contents of exercisesPractical laboratory work
      1. Milic N., Krnjaic D., Misic D., Nisavic J., Radojicic M. (2017) Microbiology and Immunology. Naucna KMD, Belgrade.
      2. Ruzica Asanin, Krnjaic D., Milic N. (2014) Manual of Microbiology and Immunology with practical exercises. Naucna KMD, Beograd.
      3. Mihajlovic BM (1983) General microbiology with basic immunology
      4. Markovic BS (1983) Microbiology II - Bacteria, fungi and yeasts
      5. Mihajlovic BM (1987) Microbiology III - Rickettsia and viruses
      6. Quinn P.J., Markey B.K., Carter M.E., Donnelly W.J.C., Leonard F.C. (2002) Veterinary Microbiology and Microbial Disease. Blackwell Science, Oxford, UK. (Original title)
      7. Hirsh D.C., Zee Y.C. (1999) Veterinary Microbiology. Blackwell Science, Massachusetts, USA. (Original title)
      8. Murphy F.A., Gibbs P.E.J., Horzinek M.C., Studdert M.J. (1999) Veterinary Virology. Academic Press, San Diego, California, USA. (Original title)
      9. Jan Tizzard (2000) Veterinary immunology. Saunders (Original title)
      Number of hours per week during the semester/trimester/year
      LecturesExercisesOTCStudy and ResearchOther classes
      Methods of teaching1. Practical laboratory work. 2. Theoretical lectures. 3. Seminary paper.
      Knowledge score (maximum points 100)
      Pre obligationsPointsFinal examPoints
      Activites during lectures10Test paper40
      Practical lessons10Oral examination20