14D2I31 - Molecular markers in analysis and detection of racial affiliation and pedigree

Course specification
Course titleMolecular markers in analysis and detection of racial affiliation and pedigree
Type of studyPhD academic studies
Lecturer (for classes)
  1. dr Zoran Stanimirović
  2. dr Jevrosima Stevanović
  3. dr Vladimir Dimitrijević
  4. dr Mila Savić
Lecturer/Associate (for practice)
    Lecturer/Associate (for OTC)
      ConditionEnrolled semester in which the subject is attended
      The goalThe subject aims to provide students the knowledge about application of molecular genetics techniques in parentage testing, kinship analysis and affirming of breed affiliation.
      The outcomeStudent should understand the role of parentage testing in animal pedigree verification and breeding programs. Finnaly, student have to be acquainted with the factors that affect the accuracy of the test for parentage determining and pedigree analysis. Student should become capable to perform parentage testing, kinship analysis and affirming of breed affiliation.
      Contents of lecturesTheoretical instructions: From Mendel to molecular markers. Types of molecular markers. Nuclear and mitochondrial DNA and correlation analyses between genetic polymorphisms and specific phenotypic characteristics. Application of data obtained by analyses of DNA polymorphisms in determination of kinship, parentage and pedigree.
      Contents of exercisesIndependent research activities: DNA isolation, amplification of conserved regions of mitochondrial DNA, amplification of nuclear DNA microsatellites (PCR, real-time PCR, RT-PCR, PCR-RFLP, LAMP), visualisation of results by gel electrophoresis and DNA sequencing, analysis of the obtained results with aimed to kinship, parentage and pedigree analyses.
      1. Đelić N, Stanimirović Z: Principi genetike, Fakultet veterinarske medicine, Univerzitet u Beogradu, Elit-Medica, Beograd, 2004. (Original title)
      2. Wilson K, Walker J: Principles and Techniques of Biochemistry and Molecular Biology, 7h edition, Cambridge University Press, 2010. (Original title)
      3. Watson JD, Baker TA, Bell SP, Gann A, Levine M, Losick R: Molecular Biology of the Gene, 7th Edition, Pearson Education, 2013. (Original title)
      4. Farkas DH, DNA from A to Z, Amer Assoc for Clinical Chemistry, 2004. (Original title)
      5. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P: Molecular Biology of the Cell, 5th ed., Garland Science, New York, 2007. (Original title)
      Number of hours per week during the semester/trimester/year
      LecturesExercisesOTCStudy and ResearchOther classes
      Methods of teachingTheoretical instruction through preparation of seminary elaborate, public interpretation and critical discussion. Independent research activities: performing experiment, analysis and discussion of the results.
      Knowledge score (maximum points 100)
      Pre obligationsPointsFinal examPoints
      Activites during lectures10Test paper70
      Practical lessonsOral examination