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14I1O08 - Biochemistry (including basic molecular biology)

Course specification
Course title Biochemistry (including basic molecular biology)
Acronym 14I1O08
Study programme Veterinary Medicine
Module
Type of study Integrated academic studies
Lecturer (for classes)
Lecturer/Associate (for practice)
Lecturer/Associate (for OTC)
    ESPB 7.0 Status obligatory
    Condition Medical Chemistry Oblik uslovljenosti
    The goal Students will understand the key principles of: the structure and functions of biological molecules , metabolic processes, storing, inheritance, and expression of genetic information. Biochemistry course will also prepare students to fully understand programs of subsequent academic years.
    The outcome After the completion of the course, students will know: principles of structure and functions of proteins, lipids, carbohydrates and nucleic acids; key metabolic pathways, mechanisms of their regulation, metabolic energy yield and needs in cells and tissues; specific metabolic adaptations of ruminants; mechanisms of storing, inheritance and expression of genetic information; theoretical and practical basis of biochemical laboratory methods.
    Contents
    Contents of lectures Amino acids; peptide bond; week interactions; primary protein structure. Structure of proteins. Collagen, myoglobin & hemoglobin, oxygen binding and transport; immunoglobulin G. Enzymes: bio-energetics, catalytic action, isozymes & multi-enzyme complexes; kinetics & regulation; coenzymes; nomenclature. Biological membranes: structure; transport across membranes, hormone signal transduction. DNA & RNA structure. Replication & reparation. RNA transcription & editing. Protein synthesis; genetic code. General metabolism & ATP synthesis. Carbohidrates: digestion, absorption & transpor; inter-conversion of hexoses; pentose phosphate pathway. Glycogen metabolism & regulation. Glucose metabolism: glycolyisis & regulation, in erythrocytes; gluconeogenesis; pyruvate & lactate axis; Cori cycle. Citric acid cycle & regulation; metabolic modifications of the CAC. Lipids: Digestion, absorption & transport of lipids; lipoproteins, fat storages & mobilization. Fatty acids: oxidation, free radicals & antioxidative protection, synthesis, ketone bodies. Triglyceride & phosphoglycerides: synthesis, biological roles. E eicosanoids. Cholesterol metabolism. Proteins: digestion, absorption & transport of amino acids; amino acids metabolism strategy. Metabolism of amino-nitrogen: transamination, desamination, urea cycle; decarboxilation & biologically active amines. Metabolism of the amino acid carbon backbone. Metabolism of heme; metabolism of nucleotides. Special features of ruminant metabolism.
    Contents of exercises Solutions, pH, buffers; proteins: chemical characteristics, concentration determination; milk; molecular separation methods: electrophoresis, chromatography, centifugation; enzymes: AP, LDH, AST, ALT; carbohydrates; lipids and cholesterol.
    Literature
    1. Mihailović MB, Jovanović IB: Biochemistry, 5th ed, Naučna, Belgrade, 2008.
    2. Jovanović IB, Valčić O. Milanović S.: Biochemistry Practicals, 3rd ed., CID, Beograd, 2015.
    3. Berg JM, Tymoczko JL, Stryer L: Biochemistry, 5th ed, WH Freeman & Co, New York, 2002. (Original title)
    4. Voyet D, Voyet J: Biochemistry, 3rd ed, Wiley, New York, 2004. (Original title)
    Number of hours per week during the semester/trimester/year
    Lectures Exercises OTC Study and Research Other classes
    4 3
    Methods of teaching Theoretical lectures with multimedia presentation, laboratory practicals, regular and appointed group consultations.
    Knowledge score (maximum points 100)
    Pre obligations Points Final exam Points
    Activites during lectures 4 Test paper 0
    Practical lessons 6 Oral examination 70
    Projects 0
    Colloquia 20
    Seminars 0