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14I2O04 - Мicrobiology w. immunology

Course specification
Course titleМicrobiology w. immunology
Acronym14I2O04
AcronymVeterinary Medicine
Module
Type of studyIntegrated academic studies
Lecturer (for classes)
  1. dr Nenad Milić
  2. dr Dejan Krnjaić
  3. dr Dušan Mišić
  4. dr Jakov Nišavić
  5. dr Marina Radojičić
Lecturer/Associate (for practice)
  1. Andrea Radalj
Lecturer/Associate (for OTC)
    ESPB10.5Status
    ConditionNone
    The goalAquisition of basic knowledge about morphology, structure, physiological and antigenic characteristics of patogenic and non-patogenic microorganisms; their ecology, resistance to antibiotics; infection; immunological reactions of organisms to antigens of microorganisms, and microbiological and immunological diagnostic methods.
    The outcomeOutcome of the course: Students should know how to collect samples, how to determine morphology and structure of microorganisms in order to identify bacteria and fungi in native and stained preparations; they should know how to perform sterilization, how to inoculate culture media, to isolate microorganisms in pure cultures and to identify them on the basis of their cultural and biochemical properties; to examine their resistance to antibiotics, to know how to perform different serological reactions and read the results; to gain tissue culture, to know how to inoculate viruses into tissue culture and to recognize cytopathic effect, to know how to inoculate chicken embryos with different kinds of viruses, rickettsia and chlamydia, to stain preparations made from alantochorial and vitelus membranes, to prepare and stain preparations obtained from brain tissue infected with rabies virus in order to detect Negri bodies.
    Contents
    Contents of lecturesIntroduction to microbiology (2), Morphological and structural characteristics of microorganisms (4), Ecology of microorganisms (4), Physiology of microorganisms (6), Prevalence of microorganisms in nature (2), Microorganisms as a means of the biological war (1), Genetics of microorganisms (3), Immunity and immunological reaction (18), Infection and defence of organism against the infection (5), obligatory colloquia (1), Special bacteriology (18), Rickettsia (1), Chlamydia (1), Colloquia (1), Mycology (3), General virology (7), Special virology (12), obligatory colloquia (1).
    Contents of exercisesThe use of the microscope in microbiology (3), Basic forms of bacteria (3), Basic morphologogical characteristics of yeast and moulds (3), Bacteriological dishes and instruments, their washing and preparation for use (3), Sterilization and devices used in sterilization (3), Bacteriological culture media (3), Making of preparations for microscopy (3), Simple staining (3), Composite staining according to Gram and Giemsa (3), Staining of capsula and bacterial spores (3), Technique of inoculation of culture media; identification of colonies and obtaining of pure cultures of bacteria (3), Cultural properties of microorganisms (3), Physiological properties of microorganisms (3), Antibiogram and examination of the resistance of bacteria to antibiotics, serological reaction (3), Colloquia (3), Staphylococcus spp., Streptococcus spp. (2), Erysipelothrix, Listeria, Corynebacterium spp. (2), Mycobacterium spp. (2), Bacillus anthracis (2), Clostridium spp. (2), Enterobacteriaceae (2), Pasteurella spp., Brucella spp. (2), Campylobacter spp., Leptospira spp. (2), Moulds (2), Chlamydia, Rickettsia (2), Application of chicken embryos in virology diagnostics (2), Viral hemagglutination and heminhibition (2), Tissue cultures and cytopathic effect (2), Herpesviruses and Rhabdoviruses (2), Colloquia (2).
    Literature
    1. Ruzica Asanin, Krnjaic D., Milic N. (2014) Manual of Microbiology and Immunology with practical exercises. Naucna KMD, Beograd.
    2. Mihajlovic BM (1983) General microbiology with basic immunology
    3. Markovic BS (1983) Microbiology II - Bacteria, fungi and yeasts
    4. Mihajlovic BM (1987) Microbiology III - Rickettsia and viruses
    Number of hours per week during the semester/trimester/year
    LecturesExercisesOTCStudy and ResearchOther classes
    65
    Methods of teachingTheoretical lectures with video bim presentation and practical teaching.
    Knowledge score (maximum points 100)
    Pre obligationsPointsFinal examPoints
    Activites during lecturesTest paper10
    Practical lessonsOral examination50
    Projects
    Colloquia40
    Seminars